Scaling and automation of a high-throughput single-cell-derived tumor sphere assay chip
نویسندگان
چکیده
منابع مشابه
High-Throughput Single-Cell Derived Sphere Formation for Cancer Stem-Like Cell Identification and Analysis
Considerable evidence suggests that many malignancies are driven by a cellular compartment that displays stem cell properties. Cancer stem-like cells (CSCs) can be identified by expression of cell surface markers or enzymatic activity, but these methods are limited by phenotypic heterogeneity and plasticity of CSCs. An alternative phenotypic methodology based on in-vitro sphere formation has be...
متن کاملQuantitative high-throughput single-cell cytotoxicity assay for T cells.
Cancer immunotherapy can harness the specificity of immune response to target and eliminate tumors. Adoptive cell therapy (ACT) based on the adoptive transfer of T cells genetically modified to express a chimeric antigen receptor (CAR) has shown considerable promise in clinical trials(1-4). There are several advantages to using CAR(+) T cells for the treatment of cancers including the ability t...
متن کاملAutomated high-throughput cell microsurgery on-chip.
Laser cell kill is an established tool for studying cells' roles during development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of Caenorhabditis elegans and software for image processing and automation, allowing for high-throughput cell ablations.
متن کاملA High Sensitivity, High Throughput, Automated Single-Cell Gel Electrophoresis (‘Comet’) DNA Damage Assay
We present an arrangement for performing, in an automated fashion, measurements of DNA damage and repair at the single cell level, using single-cell gel electrophoresis technique, more commonly known as the Comet Assay. The system is based on a standard motorised microscope and imaging components but sample scanning is performed through the capture of large, but high resolution images. Novel an...
متن کاملHigh-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay
Reactivation of latent viral reservoirs is on the forefront of HIV-1 eradication research. However, it is unknown if latency reversing agents (LRAs) increase the level of viral transcription from cells producing HIV RNA or harboring transcriptionally-inactive (latent) infection. We therefore developed a microfluidic single-cell-in-droplet (scd)PCR assay to directly measure the number of CD4+ T ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2016
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c6lc00778c